Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Rockland Immunochemicals 1X TE pH 8.0 (10 mM Tris HCl, 1.0 mM EDTA) DEPC TREATED - MB-040
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. Tris-EDTA buffer is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
Rockland Immunochemicals 1.0 M Tris HCl pH 7.6 - MB-003
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Kd Medical Inc TRIS-GLYCINE-SDS BUFFER 10X
Tris-Glycine-SDS PAGE Buffer (10X) is a concentrated solution of Tris-Glycine-SDS in distilled/deionized water, especially intended for use as the running buffer in SDS-PAGE of proteins. One 1 liter bottle.
Kd Medical Inc PBS 10X, PH 8.0
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
Kd Medical Inc TAE 50X, 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 50X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.
Kd Medical Inc PBS, 10X, PH7.4, 1 L BOTTLE
10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 1 Liter bottle.
Kd Medical Inc PBS BUFFER, 1X, PH 7.4, 500ML
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 500ml bottle.
Research Products International Corp Tris-Glycine-SDS, 10 L
Common buffer used for running SDS-PAGE gels in electrophoresis.
1X Solution Contains:
TRIS/TRIS HCl 25 mM
Glycine 0.192 M
SDS 0.1%(w/v)
![Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T32020-10000.0.JPG-250.jpg)
Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Research Products International Corp TES, Sodium Salt, 25 G
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
Teknova Tris Glycerol Buffer pH 8.0. 1000mL, Endotoxin Tested, Sterile 2 pack.
20mM Tris,25mM Sodium Chloride,2.5% Glycerol
Teknova 2M Tris-HCl, pH 7.5 Solution. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova CTAB Extraction Solution. 250mL. 4 Pack.
2.0% CTAB, 100mM Tris-HCl, pH 8.0,20mM EDTA, pH 8.0, 1.4M Sodium Chloride
Research Products International Corp PBS 100 ml Tablets 500 Tablets
PBS, 100 mL Tablets are pre-weighed tablets used to prepare 100 mL of Phosphate-Buffered Saline, a widely used buffer in biological research. Each tablet contains sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic, which dissolve in water to form an isotonic, pH-balanced solution. PBS is used in cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. These tablets ensure consistent buffer preparation, eliminate weighing steps, and save time in lab workflows. They support sample integrity and provide a stable environment for biological reactions.