Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences CHES >=99.0% (titration) | 103-47-9 | MFCD00003835 | 25G
CHES >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 25G
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 1KG
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 1KG
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 100g
Trizma base | Purity: 99.9% | Mol Wt: 121.41 | 77-86-1 | MFCD00004679 | 100g
CARTERRA INC 0.1 M MES PH 5.5
NC2232564 0.1 M MES PH 5.5
ILEX LIFE SCIENCES LLC Promed Bioscience Atelocollagen Ultrapure, Porcine Type I, Enzymatic extraction, dialyzed, lyophilized, 10 mg
Atelocollagen Ultrapure, Porcine Type I, Enzymatic Extraction, Dialyzed, Lyophilized. Characterized by rich and ultra dialysis-purified Type I collagen content in a regular fibril-like configuration that retains its superior functionality and structural properties. The proprietary pepsin enzyme-based extraction removes telopeptides at the nonhelical N and C termini, resulting in a triple helix configuration that exhibits reduced immunogenicity. APPLICATION: Biocompatible and biodegradable, suitable for many research applications, such as bioink for 3D bioprinting, in vitro cell culture, cell functional assays, etc. MANUFACTURER: Promed Bioscience Ltd. CAS NO.: 9007-34-5. PURITY: greater than 97%. SOURCE: Porcine tendon. AMINO ACID ANALYSIS: Typical profile. STERILITY TESTING: Pass (0 cfu/g). STERILIZATION METHOD: Produced under strict aseptic conditions. CELL ATTACHMENT ASSAY: Pass. CELL TOXICITY ASSAY: Pass. OTHER NAMES: Type 1 Collagen, Collagen-1.
Sigma Aldrich Fine Chemicals Biosciences MES hydrate | 1266615-59-1 | MFCD00149409 | 50 g
MES hydrate | Purity: ≥99.5% | Mol Wt: 195.24 | 1266615-59-1 | MFCD00149409 | 50 g
Sigma Aldrich Fine Chemicals Biosciences MOPS | 1132-61-2 | MFCD00006183 | 25g
MOPS | Purity: ≥99.5% (titration) | MW:209.26 | 1132-61-2 | MFCD00006183 | 25g
LAB BIOREAGENTS LowBinding IV Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
![Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660920.jpg-250.jpg)
Alkali Scientific TAE 10X Solution [TRIS Acetate EDTA 10X Solution], 1 L
TAE 10X Solution (TRIS Acetate EDTA) is a concentrated stock buffer solution used for nucleic acid electrophoresis and various molecular biology techniques. This 1-liter bottle provides a 10X stock solution that can be easily diluted to a working concentration of 1X by adding the appropriate volume of water. TAE buffer is preferred for its low ionic strength, which helps reduce background noise and allows for better resolution of nucleic acid fragments during gel electrophoresis. The inclusion of EDTA in the buffer helps protect DNA and RNA from degradation by chelating metal ions that may cause hydrolysis, making it an essential tool for safe, effective molecular analysis.
Alkali Scientific Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 20/Case
Neutralizing Buffered Peptone Water (NBPW), Sterile, Pre Enrichment Medium with Disinfectant Neutralizers, Ready to Use (Bottled) Microbiology Diluent, 400mL, 20/Case. This product is formulated for neutralizing disinfectants and enriching microbiological media. The sterile solution is ready-to-use and helps enhance the accuracy of lab tests by eliminating disinfectants in sample preparations. Suitable for use in microbiological analysis.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.0) - 500ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline, with Tween 20, pH 8.0 powder
Tris Buffered Saline, with Tween 20, pH 8.0 powder
Sigma Aldrich Fine Chemicals Biosciences HEPES 99.5 (titration), 7365-45-9, MFCD00006158
HEPES 99.5 (titration)